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nutrient agar na plates  (Thermo Fisher)


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    Thermo Fisher nutrient agar na plates
    Nutrient Agar Na Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nutrient+agar+na+plates/AGAR/pmc12154094-242-19-23
    Average 99 stars, based on 1 article reviews
    nutrient agar na plates - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: Repurposing Olive Oil Mill Wastewater into a Valuable Ingredient for Functional Bread Production.
    Article Snippet: .. The cell suspensions were then heated to 85 ◦C for 15 min, and 0.1 mL aliquots were spread on Nutrient Agar (NA) plates (Oxoid, Basingstoke, UK), which were incubated at 32 ◦C for 48 h [29]. ..

    Article Title: Application of a Quantitative Real-Time PCR Assay for Early Detection of Salmonella enterica Serovar Enteritidis on Poultry Farms During an Outbreak in New South Wales, Australia (2018–2020)
    Article Snippet: Suspect Salmonella colonies were identified as Salmonella spp. by matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) mass spectrometry using a MALDI Biotyper (Bruker, Billerica, MA, USA). .. Colonies identified as Salmonella spp. were subcultured onto nutrient agar (NA) plates (Thermo Fisher Scientific) and incubated aerobically at 37°C overnight, followed by confirmatory Salmonella latex agglutination testing (Thermo Fisher Scientific, Cat. No. DR1108A) and presumptive Salmonella serotyping with 09 polyvalent antiserum (Remel Europe, Dartford, UK, Cat. No. R30858201). ..

    Article Title: Comparative genomic analysis of ESBL-selected and non-selected Escherichia coli in Australian wastewater: Elucidating differences in diversity, antimicrobial resistance, and virulence profiles.
    Article Snippet: .. Utilising a 10 μL blue loop, the neat stock sample and the two dilutions were spread onto both Chromogenic ECC Agar Plates [Edwards, Australia] and CHROMagarT ESBL Plates [Edwards, Australia] and incubated at 37 ◦C in an O2 incubator for 16 to 18 h. Target colonies were then subcultured onto Nutrient Agar (NA) plates [ThermoFisher Scientific] and incubated overnight or for 16 to 18 h at 37 ◦C in an O2 incubator. ..

    Article Title: Microbiologically influenced corrosion on naval carbon steel inside the hull of tugboats: a case study of prevention and control.
    Article Snippet: Microbiologically influenced corrosion (MIC) has a significant cost to many industries, including naval engineering.. In this case-of-study, three tugboats developed pitting corrosion in the carbon steel of the inner hulls.. Grade A naval steel was used for the hull sheets but the inner side (corroded) showed only two protective layers of paint.

    Article Title: Repurposing Olive Oil Mill Wastewater into a Valuable Ingredient for Functional Bread Production
    Article Snippet: .. The cell suspensions were then heated to 85 °C for 15 min, and 0.1 mL aliquots were spread on Nutrient Agar (NA) plates (Oxoid, Basingstoke, UK), which were incubated at 32 °C for 48 h [ ]. ..

    Isolation:

    Article Title: Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh
    Article Snippet: .. In summary, isolated pathogens from nutrient agar (NA) plates were subcultured into Mueller–Hinton agar (Oxoid, UK) plates, and four equal wells (7 mm diameter) were cut out of each agar plate with a sterile cork borer and stainless‐steel needle. ..

    Sterility:

    Article Title: Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh
    Article Snippet: .. In summary, isolated pathogens from nutrient agar (NA) plates were subcultured into Mueller–Hinton agar (Oxoid, UK) plates, and four equal wells (7 mm diameter) were cut out of each agar plate with a sterile cork borer and stainless‐steel needle. ..

    Agglutination:

    Article Title: Application of a Quantitative Real-Time PCR Assay for Early Detection of Salmonella enterica Serovar Enteritidis on Poultry Farms During an Outbreak in New South Wales, Australia (2018–2020)
    Article Snippet: Suspect Salmonella colonies were identified as Salmonella spp. by matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) mass spectrometry using a MALDI Biotyper (Bruker, Billerica, MA, USA). .. Colonies identified as Salmonella spp. were subcultured onto nutrient agar (NA) plates (Thermo Fisher Scientific) and incubated aerobically at 37°C overnight, followed by confirmatory Salmonella latex agglutination testing (Thermo Fisher Scientific, Cat. No. DR1108A) and presumptive Salmonella serotyping with 09 polyvalent antiserum (Remel Europe, Dartford, UK, Cat. No. R30858201). ..

    Concentration Assay:

    Article Title: Microbiologically influenced corrosion on naval carbon steel inside the hull of tugboats: a case study of prevention and control.
    Article Snippet: Microbiologically influenced corrosion (MIC) has a significant cost to many industries, including naval engineering.. In this case-of-study, three tugboats developed pitting corrosion in the carbon steel of the inner hulls.. Grade A naval steel was used for the hull sheets but the inner side (corroded) showed only two protective layers of paint.

    Bacteria:

    Article Title: Microbiologically influenced corrosion on naval carbon steel inside the hull of tugboats: a case study of prevention and control.
    Article Snippet: Microbiologically influenced corrosion (MIC) has a significant cost to many industries, including naval engineering.. In this case-of-study, three tugboats developed pitting corrosion in the carbon steel of the inner hulls.. Grade A naval steel was used for the hull sheets but the inner side (corroded) showed only two protective layers of paint.



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    Survival of eight type strains of Bacillus species in rice husk biochar. A bacterial suspension was obtained by pre-culturing bacteria on a nutrient agar plate for 1 day ( a , b ), 2 days ( c , d ), or 7 days ( e , f ), followed by suspension in sterile water. Each inoculum was either nonheat-treated (NHT) ( a , c , and e ) or heat-treated (HT) at 80 °C for 1 h ( b , d , and f ) and inoculated onto biochar. The day of bacterial inoculation was designated day 0. The bacterial density in the biochar was determined after incubation at 28 °C for 7 days ( n = 3). N.D. indicates no detection. Error bar indicates standard error. Asterisks indicate significant difference ( P < 0.05) between 0 and 7 days according to t -test, and n.s. indicates no significant difference

    Journal: International Microbiology

    Article Title: Inoculation methods that enhance the survival of Bacillus strains in rice husk biochar for use as bacterial carriers

    doi: 10.1007/s10123-025-00737-5

    Figure Lengend Snippet: Survival of eight type strains of Bacillus species in rice husk biochar. A bacterial suspension was obtained by pre-culturing bacteria on a nutrient agar plate for 1 day ( a , b ), 2 days ( c , d ), or 7 days ( e , f ), followed by suspension in sterile water. Each inoculum was either nonheat-treated (NHT) ( a , c , and e ) or heat-treated (HT) at 80 °C for 1 h ( b , d , and f ) and inoculated onto biochar. The day of bacterial inoculation was designated day 0. The bacterial density in the biochar was determined after incubation at 28 °C for 7 days ( n = 3). N.D. indicates no detection. Error bar indicates standard error. Asterisks indicate significant difference ( P < 0.05) between 0 and 7 days according to t -test, and n.s. indicates no significant difference

    Article Snippet: The stock culture of Bacillus type strains was streaked onto a nutrient agar (NA) plate (Nutrient broth [BD Difco] supplemented with 1.5% agar) and cultured at 28 °C.

    Techniques: Suspension, Bacteria, Sterility, Incubation

    Effects of biochar washing on bacterial survival of eight type strains of Bacillus species. A bacterial suspension was obtained by pre-culturing bacteria in a nutrient agar plate for 1 day. The bacterial density in the inoculum of nonheat-treated (NHT) and heat-treated (HT) suspensions was determined in advance. The NHT bacterial suspension was inoculated onto unwashed biochar ( a ) or biochar after washing with reverse osmosis water ( b ). The day of bacterial inoculation was designated day 0. Survival rates ( c ) were calculated using the following formula: survival rate (%) = ( A / B ) × 100, where A is the bacterial density at 7 days and B is the bacterial density under NHT conditions at day 0. The bacterial density in the biochar was determined after incubation at 28 °C for 7 days ( n = 3). N.D. indicates no detection. Error bar indicates standard error. Survival rates were arcsine-transformed before performing statistical tests. Different letters indicate significant differences ( P < 0.05) according to the Tukey–Kramer test. Asterisks indicate significant difference ( P < 0.05) between unwashed and washed biochar according to t -test, and n.s. indicates no significant difference

    Journal: International Microbiology

    Article Title: Inoculation methods that enhance the survival of Bacillus strains in rice husk biochar for use as bacterial carriers

    doi: 10.1007/s10123-025-00737-5

    Figure Lengend Snippet: Effects of biochar washing on bacterial survival of eight type strains of Bacillus species. A bacterial suspension was obtained by pre-culturing bacteria in a nutrient agar plate for 1 day. The bacterial density in the inoculum of nonheat-treated (NHT) and heat-treated (HT) suspensions was determined in advance. The NHT bacterial suspension was inoculated onto unwashed biochar ( a ) or biochar after washing with reverse osmosis water ( b ). The day of bacterial inoculation was designated day 0. Survival rates ( c ) were calculated using the following formula: survival rate (%) = ( A / B ) × 100, where A is the bacterial density at 7 days and B is the bacterial density under NHT conditions at day 0. The bacterial density in the biochar was determined after incubation at 28 °C for 7 days ( n = 3). N.D. indicates no detection. Error bar indicates standard error. Survival rates were arcsine-transformed before performing statistical tests. Different letters indicate significant differences ( P < 0.05) according to the Tukey–Kramer test. Asterisks indicate significant difference ( P < 0.05) between unwashed and washed biochar according to t -test, and n.s. indicates no significant difference

    Article Snippet: The stock culture of Bacillus type strains was streaked onto a nutrient agar (NA) plate (Nutrient broth [BD Difco] supplemented with 1.5% agar) and cultured at 28 °C.

    Techniques: Suspension, Bacteria, Incubation, Transformation Assay

    Survival of eight type strains of Bacillus strains in biochar water extract. A bacterial suspension was obtained by pre-culturing bacteria in a nutrient agar plate for 1 day ( a – g ) or 7 days (i–p), followed by suspension in sterile water. The bacterial density in the inoculum of nonheat-treated (NHT) and heat-treated (HT) suspensions was determined in advance. The NHT bacterial suspension was inoculated in sterile water (W1, pH 6.7), high-pH sterile water (W2, pH 10.2), and biochar water extracts (biochar: water [w/v] = 0.5:10 [Ex1, pH 9.8] or 1:10 [Ex2, pH 10.2]). The day of bacterial inoculation was designated day 0. The bacterial density in the biochar was determined after incubation at 28 °C for 7 days ( n = 3). B. velezensis LMG 22478 T ( a , i ); B. amyloliquefaciens NBRC 15535 T ( b , j ); B. nakamurai CCUG 68786 T ( c , k ); B. siamensis LMG 27549 T ( d , l ); B. subtilis NBRC 13719 T ( e , m ); B. pumilus NBRC 12092 T ( f , n ); B. licheniformis NBRC 12200 T ( g , o ); and B. thuringiensis NBRC 101235 T ( h , p ). N.D. indicates no detection. Error bar indicates standard error. Different letters indicate significant differences ( P < 0.05) according to the Tukey–Kramer test

    Journal: International Microbiology

    Article Title: Inoculation methods that enhance the survival of Bacillus strains in rice husk biochar for use as bacterial carriers

    doi: 10.1007/s10123-025-00737-5

    Figure Lengend Snippet: Survival of eight type strains of Bacillus strains in biochar water extract. A bacterial suspension was obtained by pre-culturing bacteria in a nutrient agar plate for 1 day ( a – g ) or 7 days (i–p), followed by suspension in sterile water. The bacterial density in the inoculum of nonheat-treated (NHT) and heat-treated (HT) suspensions was determined in advance. The NHT bacterial suspension was inoculated in sterile water (W1, pH 6.7), high-pH sterile water (W2, pH 10.2), and biochar water extracts (biochar: water [w/v] = 0.5:10 [Ex1, pH 9.8] or 1:10 [Ex2, pH 10.2]). The day of bacterial inoculation was designated day 0. The bacterial density in the biochar was determined after incubation at 28 °C for 7 days ( n = 3). B. velezensis LMG 22478 T ( a , i ); B. amyloliquefaciens NBRC 15535 T ( b , j ); B. nakamurai CCUG 68786 T ( c , k ); B. siamensis LMG 27549 T ( d , l ); B. subtilis NBRC 13719 T ( e , m ); B. pumilus NBRC 12092 T ( f , n ); B. licheniformis NBRC 12200 T ( g , o ); and B. thuringiensis NBRC 101235 T ( h , p ). N.D. indicates no detection. Error bar indicates standard error. Different letters indicate significant differences ( P < 0.05) according to the Tukey–Kramer test

    Article Snippet: The stock culture of Bacillus type strains was streaked onto a nutrient agar (NA) plate (Nutrient broth [BD Difco] supplemented with 1.5% agar) and cultured at 28 °C.

    Techniques: Suspension, Bacteria, Sterility, Incubation

    Long-term changes in bacterial density ( a ) and survival rate ( b ) of eight type strains of Bacillus strain in biochar. A bacterial suspension was obtained by pre-culturing bacteria in a nutrient agar plate for over 7 days, followed by suspension in sterile water. The bacterial density in the inoculum of nonheat-treated (NHT) and heat-treated (HT) suspensions was determined in advance. The HT bacterial suspension was inoculated onto unwashed biochar. The day of bacterial inoculation was designated day 0. The bacterial density in the biochar was determined after incubation at 25 °C for 2, 5, and 12 weeks ( n = 6). Survival rates were calculated using the following formula: survival rate (%) = ( A / B ) × 100, where A is the bacterial density at 2, 5, or 12 weeks and B is the bacterial density under NHT conditions at day 0. Error bar indicates standard error. Asterisks indicate significant differences ( P < 0.05) from bacterial densities of NHT inoculation at day 0 in each Bacillus strain according to Dunnett’s test. Effect modifications ( P < 0.001) in among Bacillus species, periods, and combination of Bacillus species and periods in bacterial densities in the biochar were detected by two-way ANOVA

    Journal: International Microbiology

    Article Title: Inoculation methods that enhance the survival of Bacillus strains in rice husk biochar for use as bacterial carriers

    doi: 10.1007/s10123-025-00737-5

    Figure Lengend Snippet: Long-term changes in bacterial density ( a ) and survival rate ( b ) of eight type strains of Bacillus strain in biochar. A bacterial suspension was obtained by pre-culturing bacteria in a nutrient agar plate for over 7 days, followed by suspension in sterile water. The bacterial density in the inoculum of nonheat-treated (NHT) and heat-treated (HT) suspensions was determined in advance. The HT bacterial suspension was inoculated onto unwashed biochar. The day of bacterial inoculation was designated day 0. The bacterial density in the biochar was determined after incubation at 25 °C for 2, 5, and 12 weeks ( n = 6). Survival rates were calculated using the following formula: survival rate (%) = ( A / B ) × 100, where A is the bacterial density at 2, 5, or 12 weeks and B is the bacterial density under NHT conditions at day 0. Error bar indicates standard error. Asterisks indicate significant differences ( P < 0.05) from bacterial densities of NHT inoculation at day 0 in each Bacillus strain according to Dunnett’s test. Effect modifications ( P < 0.001) in among Bacillus species, periods, and combination of Bacillus species and periods in bacterial densities in the biochar were detected by two-way ANOVA

    Article Snippet: The stock culture of Bacillus type strains was streaked onto a nutrient agar (NA) plate (Nutrient broth [BD Difco] supplemented with 1.5% agar) and cultured at 28 °C.

    Techniques: Suspension, Bacteria, Sterility, Incubation